Mouse Myosin Light Chain Kinase - Smooth Muscle

Mfr.No.

E2281Mo

Sensitivity

5.41ng/L

Detection Range

10-2000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Cardiac-MLCK;Cardiac-MyBP-C-associated Ca/CaM kinase;D830007F02Rik;MLCK;MYLK;Myosin light chain kinase 3

Molecular Weight

86372

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2281Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	494.55	24.7	5
Intra-Assay	2	18	678.78	24.11	3.55
Intra-Assay	3	18	208.73	12.51	5.99

Background

Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.

Protocol

These standard curves of E2281Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.119	2.18	2.122
	2.241
600ng/L	1.174	1.187	1.129
	1.2
300ng/L	0.774	0.774	0.715
	0.774
150ng/L	0.455	0.443	0.384
	0.43
75ng/L	0.214	0.208	0.149
	0.201
0ng/L	0.064	0.059	0
	0.054

Gene ID	213435
UniProt ID	Q3UIZ8

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110092	E2281Mo	48T	$320		Add to Cart
BHE12110092	E2281Mo	96T	$458		Add to Cart

Mouse Myosin Light Chain Kinase - Smooth Muscle - MYLK ELISA Kit

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