Mouse Myoglobin,MYO/MB ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08050m

Alternative Names

myoglobin, MGC13548, PVALB, OTTHUMP00000197922

Reactivity

Mouse

Detection Range

15.6 ng/mL-1000 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	96-104
EDTA plasma (n=4)	90	86-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-103
1:2	Average %	90
	Range %	85-103
1:4	Average %	93
	Range %	88-100
1:8	Average %	89
	Range %	82-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

3.9 ng/mL

Limit Of Detection

450 nm

UniProt ID	P04247
Antigen	Myoglobin

Applications

ELISA

Research Area

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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