Mouse Myeloblastin - PRTN3 ELISA Kit

Mfr.No.

E2276Mo

Sensitivity

0.052U/mL

Detection Range

0.1-40U/mL

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

MBN;MBT;Myeloblastin;NP4;NP-4;P29;PR3;PR-3;Proteinase 3;PRTN 3;PRTN3

Molecular Weight

27626

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2276Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	10.2	0.67	6.5
Intra-Assay	2	18	4	0.27	6.8
Intra-Assay	3	18	1.8	0.09	5

Background

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.

Protocol

These standard curves of E2276Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24U/mL	2.28	2.289	2.253
	2.298
12U/mL	1.744	1.85	1.814
	1.957
6U/mL	1.183	1.179	1.143
	1.175
3U/mL	0.733	0.725	0.689
	0.718
1.5U/mL	0.371	0.4	0.364
	0.429
0U/mL	0.044	0.036	0
	0.028

Gene ID	19152
UniProt ID	Q61096

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110087	E2276Mo	48T	$320		Add to Cart
BHE12110087	E2276Mo	96T	$458		Add to Cart

Mouse Myeloblastin - PRTN3 ELISA Kit

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