Mouse Muscarinic Acetylcholine Receptor M1

Mfr.No.

E2272Mo

Sensitivity

5.09ng/L

Detection Range

10-2000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

AChR;CHRM 1;CHRM1;HM1;Muscarinic acetylcholine receptor M1

Molecular Weight

51379

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2272Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	224.9	11.47	5.1
Intra-Assay	2	18	505.3	20.53	4.1
Intra-Assay	3	18	811.8	21.38	2.6

Background

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium Channels through the action of G proteins. Primary transducing effect is Pi turnover.

Protocol

These standard curves of E2272Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.429	2.397	2.366
	2.364
600ng/L	1.409	1.363	1.333
	1.318
300ng/L	0.738	0.659	0.629
	0.581
150ng/L	0.422	0.387	0.357
	0.352
75ng/L	0.116	0.143	0.112
	0.169
0ng/L	0.018	0.031	0
	0.043

Gene ID	12669
UniProt ID	P12657

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110083	E2272Mo	48T	$320		Add to Cart
BHE12110083	E2272Mo	96T	$458		Add to Cart

Mouse Muscarinic Acetylcholine Receptor M1 - CHRM1 ELISA Kit

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