Mouse Mucin-5 subtype AC,MUC5AC ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11040m

Sensitivity

1.172 ng/mL

Detection Range

4.688 ng/mL-300 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Mucin-5 subtype AC,MUC5AC

Recovery

The recovery of pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	98	92-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	84-105
1:2	Average %	93
	Range %	89-99
1:4	Average %	95
	Range %	89-101
1:8	Average %	95
	Range %	83-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	MUC5AC

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice /,PLOS ONE,2012 7(7):e41088. A Dissociated Glucocorticoid Receptor Modulator Reduces Airway Hyperresponsiveness and Inflammation in a Mouse Model of Asthma /,The Joual of Immunology,2012 Apr 1;188(7):3478-87. The immune profile associated with acute allergic asthma accelerates clearance of influenza virus Samarasinghe AE et al,Immunol Cell Biol,Jan 28. Chronic intermittent hypoxiaDecreases pulmonary clearance of 99mTc-labelled particulate matter in mice Fu C.et al,Am J Transl Res,Jun 15

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