Matrices listed below were spiked with certain level of Mt1 and the recovery rates were calculated by comparing the measured value to the expected amount of Mt1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-103
98
EDTA plasma(n=5)
85-104
96
heparin plasma(n=5)
91-99
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mt1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Mt1 ELISA Kit allows for the in vitro quantitative determination of Mt1 concentrations in serum, plasma, tissue homogenates and other biological fluids.