Matrices listed below were spiked with certain level of Mstn and the recovery rates were calculated by comparing the measured value to the expected amount of Mstn in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
100
EDTA plasma(n=5)
88-101
95
heparin plasma(n=5)
88-98
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mstn and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
MSTN ELISA Kit allows for the in vitro quantitative determination of Mstn concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse MSTN(MSTN/Growth differentiation factor 8) ELISA Kit