Mouse MPO/Myeloperoxidase ELISA Kit PicoKine®

Mfr.No.

EK0943

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.034	0.070	0.108	0.184	0.427	0.660	1.378	2.095

Description

Mouse MPO/Myeloperoxidase ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mpo in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Myeloperoxidase;MPO;1.11.2.2;Myeloperoxidase light chain;Myeloperoxidase heavy chain;Mpo;

Molecular Weight

81182 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M16-T718

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse MPO

Subcellular Localization

Lysosome.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	354	1809	5328	362	1718	5171
Standard deviation	19.11	115.77	266.4	346.457	137.44	346.45
CV (%)	5.4%	6.4%	5%	6.9%	8%	6.7%

Protein Function

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (By similarity). .

Background

Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	354	346	367	309	344	21.55	6.2%
Sample 2	1809	1947	1689	1851	1824	92.61	5%
Sample 3	5328	4954	4733	4680	4923	255.01	5.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	17523
Gene Name	MPO
UniProt ID	P11247
Protein Name	Myeloperoxidase

Applications	ELISA
Research Area	Cancer,Cancer Metabolism,Cardiovascular,Immunology,Innate Immunity,Macrophage/Inflammation,Metabolism,Pathways and Processes,Redox Metabolism,Vasculature

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000716	EK0943	96 wells/kit	$499		Add to Cart

Mouse MPO/Myeloperoxidase ELISA Kit PicoKine®

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