Mouse monocyte chemotactic protein 3,MCP-3 ELISA kit

Mouse monocyte chemotactic protein 3,MCP-3 ELISA kit

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Mfr.No.

CSB-E07426m

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 7, FIC, MARC, MCP-3, MCP3, MGC138463, MGC138465, NC28, SCYA6, SCYA7, monocyte chemoattractant protein 3, monocyte chemotactic protein 3, small inducible cytokine A7 (monocyte chemotactic protein 3)

Recovery

The recovery of rat SDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	96	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	99
	Range %	93-105
1:2	Average %	96
	Range %	91-100
1:4	Average %	94
	Range %	89-98
1:8	Average %	85
	Range %	81-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.562	2.463	2.513	2.388
100	2.113	1.994	2.054	1.929
50	1.520	1.491	1.506	1.381
25	1.053	1.044	1.049	0.924
12.5	0.614	0.623	0.619	0.494
6.25	0.361	0.370	0.366	0.241
3.12	0.239	0.243	0.241	0.116
0	0.124	0.125	0.125	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q03366

Applications	ELISA
Research Area	CCL7

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.