Mouse MMP-8/Neutrophil collagenase ELISA Kit PicoKine®

Mfr.No.

EK1423

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.009	0.025	0.055	0.126	0.246	0.499	0.907	1.880

Description

Mouse MMP-8 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mmp8 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neutrophil collagenase;3.4.24.34;Collagenase 2;Matrix metalloproteinase-8;MMP-8;Mmp8;

Molecular Weight

53126 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: F21-S465

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse MMP-8

Subcellular Localization

Cytoplasmic granule. Secreted, extracellular space, extracellular matrix. Stored in intracellular granules and released during inflammatory conditions.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	296	1549	4681	323	1418	4367
Standard deviation	18.64	106.88	204.4%6	26.48	120.53	231.45
CV (%)	8.5%%	6.9%	4.4%	8.2%	8.5%	5.3%

Protein Function

Can degrade fibrillar type I, II, and III collagens. May play a role in the degradation of collagen fibers during uterine involution.

Background

Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	296	312	269	309	296	16.97	5.7%
Sample 2	1549	1324	1494	1407	1443	85.57	5.9%
Sample 3	4681	4754	5080	4797	4828	151.28	3.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	17394
Gene Name	Mmp8
UniProt ID	O70138
Protein Name	Neutrophil collagenase
Tissue Specificity	Neutrophils. Expressed in uterus. Low levels in kidney and muscle.

Applications	ELISA
Research Area	Angiogenesis,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Metalloprotease,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction,Tumor Biomarkers,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001156	EK1423	96 wells/kit	$499		Add to Cart

Mouse MMP-8/Neutrophil collagenase ELISA Kit PicoKine®

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