Mixed lineage kinase domain-like protein ELISA Kit, Mlkl ELISA Kit
Reactivity
Mouse
Detection Method
Double Antibody
Detection Range
31.25-2000pg/ml
Sample Type
Cell lysate, Tissue lysate, Other liquid samples
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Mlkl and the recovery rates were calculated by comparing the measured value to the expected amount of Mlkl in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
96
EDTA plasma(n=5)
82-104
90
heparin plasma(n=5)
83-100
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mlkl and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-104%
84-102%
94-104%
EDTA plasma(n=5)
90-97%
92-101%
94-97%
heparin plasma(n=5)
91-100%
92-100%
92-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q9D2Y4
Applications
ELISA
Applications Notes
MlklELISA Kit allows for the in vitro quantitative determination of Mlkl concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Mlkl(Mixed lineage kinase domain-like protein)ELISA Kit