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Home / ELISA Kit

Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit

SKU : BHE13705388

TIM44, a peripheral inner membrane protein, tethers mitochondrial HSP70 to the import channel. A model peptide substrate rapidly released mitochondrial HSP70 from TIM44, even in the absence of ATP hydrolysis. In vivo, the analogous interaction of translocating polypeptide would release mitochondrial HSP70 from the channel.
TIMM44 is hydrophilic and contains no hydrophobic membrane-spanning segments. The N terminus has a coiled-coil motif and a matrix-targeting segment with 6 positively and no negatively charged residues. Western blot analysis showed that TIMM44 is present in membranes as well as matrix and is most likely a 45-kD peripheral membrane protein loosely associated with the inner membrane of mitochondria.

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Mfr.No.	KTE70228
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMM44.
Alternative Names	TIMM44; DKFZp686H05241; TIM44; mitochondrial inner membrane translocase; translocase of inner mitochondrial membrane 44
Detection Method	Colorimetric
Kit Components	Mouse Mitochondrial import inner membrane translocase subunit TIM44 microplate Mouse Mitochondrial import inner membrane translocase subunit TIM44 standard Mouse Mitochondrial import inner membrane translocase subunit TIM44 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TIMM44. No significant cross-reactivity or interference between Mouse TIMM44 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TIM44, a peripheral inner membrane protein, tethers mitochondrial HSP70 to the import channel. A model peptide substrate rapidly released mitochondrial HSP70 from TIM44, even in the absence of ATP hydrolysis. In vivo, the analogous interaction of translocating polypeptide would release mitochondrial HSP70 from the channel. TIMM44 is hydrophilic and contains no hydrophobic membrane-spanning segments. The N terminus has a coiled-coil motif and a matrix-targeting segment with 6 positively and no negatively charged residues. Western blot analysis showed that TIMM44 is present in membranes as well as matrix and is most likely a 45-kD peripheral membrane protein loosely associated with the inner membrane of mitochondria.
Notes	This Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMM44 in samples. An antibody specific for TIMM44 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMM44 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMM44 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMM44 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	21856

Applications	ELISA
Applications Notes	This Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMM44 in samples. An antibody specific for TIMM44 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMM44 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMM44 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMM44 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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