Mouse MIP-2 ELISA Kit PicoKine®

Mfr.No.

EK0452

Sensitivity

<5 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.001	0.130	0.249	0.425	0.811	1.227	1.833	2.039

Description

Mouse MIP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cxcl2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-X-C motif chemokine 2;Macrophage inflammatory protein 2;MIP2;Cxcl2;Mip-2, Mip2, Scyb2;

Molecular Weight

10621 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A28-N100

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse MIP-2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	39	230	434	37	220	409
Standard deviation	1.79	17.94	17.79	1.85	18.7	22.9
CV (%)	4.6%	7.8%	4.1%	5%	8.5%	5.6%

Protein Function

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.

Background

MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells. The standard used in this kit is recombinant mouse MIP-2 (A28-N100), consisting of 73 amino acids with the molecular mass of 8KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	39	34	40	41	38	2.69	7%
Sample 2	230	234	204	215	220	11.98	5.4%
Sample 3	434	404	419	468	431	23.72	5.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20310
Gene Name	CXCL2
UniProt ID	P10889
Protein Name	C-X-C motif chemokine 2

Applications	ELISA
Research Area	Chemokines,Immunology,Innate Immunity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000447	EK0452	96 wells/kit	$499		Add to Cart

Mouse MIP-2 ELISA Kit PicoKine®

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