Mouse Midkine(MDK) ELISA kit

Mfr.No.

CSB-EL013624MO

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

midkine (neurite growth-promoting factor 2), FLJ27379, MK, NEGF2, OTTHUMP00000197041, OTTHUMP00000197042, OTTHUMP00000197045, midkine

Recovery

The recovery of human KNG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	108	99-112
EDTA plasma (n=4)	94	86-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human KNG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	99
	Range %	90-103
1:200	Average %	105
	Range %	98-109
1:400	Average %	92
	Range %	83-97
1:800	Average %	95
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P12025

Applications	ELISA
Research Area	MDK

Storage	Store at 2-8°C. Please refer to protocol.

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