Mouse Midkine(MDK) ELISA kit

Mfr.No.

CSB-EL013624MO

Alternative Names

midkine (neurite growth-promoting factor 2), FLJ27379, MK, NEGF2, OTTHUMP00000197041, OTTHUMP00000197042, OTTHUMP00000197045, midkine

Reactivity

Mouse

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human KNG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	108	99-112
EDTA plasma (n=4)	94	86-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human KNG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	99
	Range %	90-103
1:200	Average %	105
	Range %	98-109
1:400	Average %	92
	Range %	83-97
1:800	Average %	95
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

UniProt ID	P12025
Antigen	midkine (neurite growth-promoting factor 2)

Applications

ELISA

Research Area

MDK

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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