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Home / ELISA Kit

Mouse Microtubule-associated protein 1S (MAP1S) ELISA Kit

SKU : BHE13706292

Using the SEC1 homology domain of LRPPRC as probe in a yeast 2-hybrid screen, Liu and McKeehan (2002) cloned VCY2IP1 (BPY2IP1), which they called C19ORF5, from a liver cDNA library.The deduced 672-amino acid protein is proline-rich (12.8%) and contains an arginine-rich potential RNA-binding motif. VCY2IP1 shares significant homology with several microtubule-associated proteins. Northern blot analysis revealed a major transcript of about 3 kb in all 12 tissues tested. Additional less-abundant transcripts were detected in some tissues.The International Radiation Hybrid Mapping Consortium mapped the VCY2IP1 gene to chromosome 20.

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Mfr.No.	KTE71133
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Microtubule-associated protein 1S (MAP1S) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1S.
Alternative Names	MAP1S; BPY2IP1; C19orf5; FLJ10669; MAP8; MGC133087; VCY2IP-1; VCY2IP1; BPY2 interacting protein 1; VCY2 interacting protein 1; microtubule-associated protein 8
Detection Method	Colorimetric
Kit Components	Mouse Microtubule-associated protein 1S microplate Mouse Microtubule-associated protein 1S standard Mouse Microtubule-associated protein 1S detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Microtubule-associated protein 1S (MAP1S) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAP1S. No significant cross-reactivity or interference between Mouse MAP1S and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Using the SEC1 homology domain of LRPPRC as probe in a yeast 2-hybrid screen, Liu and McKeehan (2002) cloned VCY2IP1 (BPY2IP1), which they called C19ORF5, from a liver cDNA library.The deduced 672-amino acid protein is proline-rich (12.8%) and contains an arginine-rich potential RNA-binding motif. VCY2IP1 shares significant homology with several microtubule-associated proteins. Northern blot analysis revealed a major transcript of about 3 kb in all 12 tissues tested. Additional less-abundant transcripts were detected in some tissues.The International Radiation Hybrid Mapping Consortium mapped the VCY2IP1 gene to chromosome 20.
Notes	This Mouse Microtubule-associated protein 1S (MAP1S) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1S in samples. An antibody specific for MAP1S has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1S present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1S is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1S bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	270058

Applications	ELISA
Applications Notes	This Mouse Microtubule-associated protein 1S (MAP1S) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1S in samples. An antibody specific for MAP1S has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1S present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1S is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1S bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13706292	KTE71133	48 T	$339		Add to Cart
BHE13706292	KTE71133	96 T	$589		Add to Cart
BHE13706292	KTE71133	96 Tx5	$2629		Add to Cart
BHE13706292	KTE71133	96 Tx50	$24569		Add to Cart

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