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Home / ELISA Kit

Mouse Methemoglobin (MHB) ELISA Kit

SKU : BHE13706190

Methemoglobin is a form of the oxygen-carrying metalloprotein hemoglobin, in which the iron in the heme group is in the Fe3+ (ferric) state, not the Fe2+ (ferrous) of normal hemoglobin. Methemoglobin cannot bind oxygen, unlike oxyhemoglobin. It is bluish chocolate-brown in color. In human blood a trace amount of methemoglobin is normally produced spontaneously, but when present in excess the blood becomes abnormally dark bluish brown. The NADH-dependent enzyme methemoglobin reductase (diaphorase I) is responsible for converting methemoglobin back to hemoglobin.

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Mfr.No.	KTE71031
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Methemoglobin (MHB) ELISA Kit employs a two-site sandwich ELISA to quantitate MHB.
Alternative Names	MHB
Detection Method	Colorimetric
Kit Components	Mouse Methemoglobin microplate Mouse Methemoglobin standard Mouse Methemoglobin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Methemoglobin (MHB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MHB. No significant cross-reactivity or interference between Mouse MHB and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Methemoglobin is a form of the oxygen-carrying metalloprotein hemoglobin, in which the iron in the heme group is in the Fe3+ (ferric) state, not the Fe2+ (ferrous) of normal hemoglobin. Methemoglobin cannot bind oxygen, unlike oxyhemoglobin. It is bluish chocolate-brown in color. In human blood a trace amount of methemoglobin is normally produced spontaneously, but when present in excess the blood becomes abnormally dark bluish brown. The NADH-dependent enzyme methemoglobin reductase (diaphorase I) is responsible for converting methemoglobin back to hemoglobin.
Notes	This Mouse Methemoglobin (MHB) ELISA Kit employs a two-site sandwich ELISA to quantitate MHB in samples. An antibody specific for MHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Methemoglobin (MHB) ELISA Kit employs a two-site sandwich ELISA to quantitate MHB in samples. An antibody specific for MHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13706190	KTE71031	48 T	$359		Add to Cart
BHE13706190	KTE71031	96 T	$619		Add to Cart
BHE13706190	KTE71031	96 Tx5	$2809		Add to Cart
BHE13706190	KTE71031	96 Tx50	$26209		Add to Cart

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