Mouse Membrane primary amine oxidase(AOC3) ELISA kit

Mouse Membrane primary amine oxidase(AOC3) ELISA kit

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Mfr.No.

CSB-EL001855MO

Sensitivity

0.31 ng/mL

Detection Range

1.25 ng/mL-80 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

amine oxidase, copper containing 3 (vascular adhesion protein 1), HPAO, SSAO, VAP-1, VAP1, amine oxidase, copper containing 3, copper amine oxidase, semicarbazide-sensitive amine oxidase, vascular adhesion protein 1

Recovery

The recovery of canine trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	87-107
EDTA plasma (n=4)	97	86-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	85-106
1:2	Average %	93
	Range %	85-105
1:4	Average %	95
	Range %	82-102
1:8	Average %	95
	Range %	84-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
2500	2.263	2.319	2.291	2.162
1250	1.861	1.954	1.908	1.779
625	1.365	1.372	1.369	1.240
312	0.874	0.898	0.886	0.757
156	0.592	0.583	0.588	0.459
78	0.307	0.298	0.303	0.174
39	0.232	0.246	0.239	0.110
0	0.127	0.131	0.129	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O70423

Applications	ELISA
Research Area	AOC3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506241	CSB-EL001855MO	96T	$595	Add to Cart
BHE10506241	CSB-EL001855MO	5x96T	$2856	Add to Cart
BHE10506241	CSB-EL001855MO	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.