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Home / ELISA Kit

Mouse Melanoma-derived Growth Regulatory Protein - MIA ELISA Kit

SKU : BHE12110055

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

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Bioassay Technology Laboratory

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Mfr.No.

E2244Mo

Sensitivity

0.97ng/L

Detection Range

2-600ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Cartilage-derived retinoic acid-sensitive protein;CD-RAP;Melanoma inhibitory activity protein;Melanoma-derived growth regulatory protein;MIA

Molecular Weight

14593

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2244Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	39.9	2.12	5.3
Intra-Assay	2	18	170.1	10.33	6.1
Intra-Assay	3	18	73.1	4.04	5.5

Background

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

Protocol

These standard curves of E2244Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	2.294	2.298	2.265
	2.302
160ng/L	1.823	1.806	1.773
	1.79
80ng/L	1.085	1.138	1.104
	1.19
40ng/L	0.624	0.65	0.617
	0.676
20ng/L	0.382	0.371	0.338
	0.361
0ng/L	0.039	0.033	0
	0.027

Gene ID	12587
UniProt ID	Q61865

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110055	E2244Mo	48T	$320		Add to Cart
BHE12110055	E2244Mo	96T	$458		Add to Cart

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