Mouse Melanoma-derived growth regulatory protein(MIA) ELISA kit

Mfr.No.

CSB-EL013798MO

Sensitivity

1.17 pg/mL

Detection Range

4.7 pg/mL-300 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

melanoma inhibitory activity, CD-RAP

Recovery

The recovery of human ILK-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	87	83-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ILK-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	97
	Range %	92-101
1:4	Average %	90
	Range %	86-94
1:8	Average %	105
	Range %	100-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q61865

Applications	ELISA
Research Area	MIA

Storage	Store at 2-8°C. Please refer to protocol.

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