Matrices listed below were spiked with certain level of MDA and the recovery rates were calculated by comparing the measured value to the expected amount of MDA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-98
95
EDTA plasma(n=5)
87-103
94
heparin plasma(n=5)
88-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MDA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
85-100%
93-101%
EDTA plasma(n=5)
87-97%
84-101%
84-99%
heparin plasma(n=5)
86-100%
85-97%
80-93%
Sensitivity
< 4.688ng/ml
Notes
For Research Use Only
Antigen
MDA
Applications
ELISA
Applications Notes
MDA ELISA Kit allows for the in vitro quantitative determination of MDA concentrations in serum, plasma, tissue homogenates and other biological fluids.