Mouse matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

Mfr.No.

CSB-E04676m

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), CLG4, CLG4A, MMP-II, MONA, TBE-1, collagenase type IV-A, matrix metalloproteinase 2, matrix metalloproteinase-II, neutrophil gelatinase

Recovery

The recovery of rat MMP-14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	98	95-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-95
1:2	Average %	95
	Range %	90-102
1:4	Average %	97
	Range %	95-107
1:8	Average %	101
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.288	2.289	2.289	2.092
1500	1.736	1.752	1.744	1.547
750	1.118	1.097	1.108	0.911
375	0.674	0.727	0.701	0.504
187.5	0.455	0.433	0.444	0.247
93.75	0.336	0.331	0.334	0.137
46.88	0.265	0.256	0.261	0.064
0	0.205	0.189	0.197	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P33434

Applications	ELISA
Research Area	MMP2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506226	CSB-E04676m	96T	$495	Add to Cart
BHE10506226	CSB-E04676m	5x96T	$2376	Add to Cart
BHE10506226	CSB-E04676m	10x96T	$4702.50	Add to Cart

Mouse matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

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