Mouse matrix metalloproteinase 10,MMP-10 ELISA kit

Mouse matrix metalloproteinase 10,MMP-10 ELISA kit

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Mfr.No.

CSB-E07415m

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

matrix metallopeptidase 10 (stromelysin 2), SL-2, STMY2, matrix metalloprotease 10, matrix metalloproteinase 10, matrix metalloproteinase 10 (stromelysin 2), stromelysin 2, transin 2

Recovery

The recovery of rat RLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	100-110
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat RLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	89
	Range %	84-93
1:4	Average %	92
	Range %	88-96
1:8	Average %	97
	Range %	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
150	2.643	2.487	2.565	2.439
75	2.097	2.236	2.167	2.041
37.5	1.468	1.551	1.510	1.384
18.75	0.907	0.945	0.926	0.800
9.4	0.583	0.617	0.600	0.474
4.7	0.384	0.401	0.393	0.267
2.4	0.235	0.252	0.244	0.118
0	0.121	0.130	0.126

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O55123

Applications	ELISA
Research Area	MMP10

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506215	CSB-E07415m	96T	$615	Add to Cart
BHE10506215	CSB-E07415m	5x96T	$2952	Add to Cart
BHE10506215	CSB-E07415m	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.