Mouse matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) ELISA kit

Mfr.No.

CSB-E07409m

Alternative Names

matrix metallopeptidase 7 (matrilysin, uterine), MMP-7, MPSL1, PUMP-1, matrin, matrix metalloproteinase 7, matrix metalloproteinase 7 (matrilysin, uterine), uterine matrilysin

Reactivity

Mouse

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of bovine PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	96	90-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-99
1:2	Average %	96
	Range %	90-110
1:4	Average %	90
	Range %	83-99
1:8	Average %	95
	Range %	85-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.626	2.603	2.615	2.498
1000	2.365	2.325	2.345	2.228
500	1.986	1.992	1.989	1.872
250	1.502	1.486	1.494	1.377
125	0.841	0.835	0.838	0.721
62.5	0.486	0.474	0.480	0.363
31.25	0.371	0.375	0.373	0.256
0	0.115	0.119	0.117	?

Species

Mus musculus

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q10738
Antigen	matrix metallopeptidase 7 (matrilysin, uterine)

Applications

ELISA

Research Area

MMP7

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506214	CSB-E07409m	96T	$615	Add to Cart
BHE10506214	CSB-E07409m	5x96T	$2952	Add to Cart
BHE10506214	CSB-E07409m	10x96T	$5842.50	Add to Cart

Mouse matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) ELISA kit

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