Mouse Matrilin-2 ELISA Kit PicoKine®

Mfr.No.

EK2021

Sensitivity

<50 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.044	0.132	0.212	0.346	0.591	1.063	1.659	2.189

Description

Mouse Matrilin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Matn2 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Matrilin-2; Matn2

Immunogen

Expression system for standard: NS0; Immunogen sequence: R24-R956

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Matrilin-2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	178	657	2396	181	685	2417
Standard deviation	8.01	37.45	110.22	9.23	40.42	178.86
CV (%)	4.5%	5.7%	4.6%	5.1%	5.9%	7.4%

Protein Function

Involved in matrix assembly.

Background

Matrilin-2 is a protein that in humans is encoded by the MATN2 gene. This gene is mapped to 15; 15 B3.1. This gene encodes a member of the von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains five von Willebrand factor A domains. The specific function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	178	194	184	185	185	5.71	3.0%
Sample 2	657	670	600	591	629	34.45	5.4%
Sample 3	2396	2289	2414	2332	2357	50.04	2.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	17181
Gene Name	Matn2
UniProt ID	O08746
Protein Name	Matrilin-2
Tissue Specificity	Detected in a variety of organs, including calvaria, uterus, heart and brain, as well as fibroblast and osteoblast cell lines.

Applications	ELISA
Research Area	Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001527	EK2021	96 wells/kit	$499		Add to Cart

Mouse Matrilin-2 ELISA Kit PicoKine®

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