Mouse Mast Cell Tryptase(MCT) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14326m

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Mast Cell Tryptase(MCT)

Recovery

The recovery of rat KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	95-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-95
1:400	Average %	103
	Range %	99-105
1:800	Average %	91
	Range %	85-97
1:1600	Average %	96
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q02844

Applications	ELISA
Research Area	MCT

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Rapid Mast Cell Generation from Gata2 Reporter Pluripotent Stem Cells Mari-Liis Kauts.et al,STEM CELL REPORTS,August

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