Mouse Mannan-binding lectin serine protease 2(MASP2) ELISA kit

Mfr.No.

CSB-EL013510MO

Sensitivity

31.2 pg/mL

Detection Range

125 pg/mL-8000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

mannan-binding lectin serine peptidase 2, MAP19, MASP-2, sMAP, MBL-associated plasma protein of 19 kD, MBL-associated serine protease 2, mannan-binding lectin serine protease 2, small MBL-associated protein

Recovery

The recovery of human KLK 7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-108
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	92-108
1:2	Average %	94
	Range %	91-97
1:4	Average %	92
	Range %	86-98
1:8	Average %	97
	Range %	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.708	2.607	2.658	2.514
20	1.865	1.764	1.815	1.671
10	1.217	1.206	1.212	1.068
5	0.811	0.840	0.826	0.682
2.5	0.458	0.447	0.453	0.309
1.25	0.322	0.311	0.317	0.173
0.625	0.235	0.232	0.234	0.090
0	0.144	0.143	0.144	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q91WP0

Applications	ELISA
Research Area	MASP2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506207	CSB-EL013510MO	96T	$695	Add to Cart
BHE10506207	CSB-EL013510MO	5x96T	$3336	Add to Cart
BHE10506207	CSB-EL013510MO	10x96T	$6602.50	Add to Cart

Mouse Mannan-binding lectin serine protease 2(MASP2) ELISA kit

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