Mouse Major Centromere Autoantigen B

Mfr.No.

E2232Mo

Sensitivity

7.45ng/L

Detection Range

15-3000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CENPB;CENP-B;Centromere protein B;Major centromere autoantigen B

Molecular Weight

65381

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2232Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	488.6	27.34	5.6
Intra-Assay	2	18	1089.3	25.59	2.3
Intra-Assay	3	18	837.4	37.45	4.5

Background

Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

Protocol

These standard curves of E2232Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.978	1.968	1.929
	1.958
800ng/L	0.865	0.914	0.875
	0.962
400ng/L	0.481	0.449	0.41
	0.416
200ng/L	0.283	0.283	0.244
	0.283
100ng/L	0.148	0.15	0.111
	0.152
0ng/L	0.039	0.039	0
	0.04

Gene ID	12616
UniProt ID	P27790

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110043	E2232Mo	48T	$320		Add to Cart
BHE12110043	E2232Mo	96T	$458		Add to Cart

Mouse Major Centromere Autoantigen B - CENPB ELISA Kit

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