Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Recovery
Matrices listed below were spiked with certain level of Magee1 and the recovery rates were calculated by comparing the measured value to the expected amount of Magee1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-96
91
EDTA plasma(n=5)
85-102
93
heparin plasma(n=5)
89-102
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Magee1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-103%
89-105%
85-105%
EDTA plasma(n=5)
89-100%
90-96%
84-98%
heparin plasma(n=5)
80-100%
87-99%
81-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q6PCZ4
Applications
ELISA
Applications Notes
Magee1ELISA Kit allows for the in vitro quantitative determination of Magee1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Magee1 (Melanoma-associated antigen E1) ELISA Kit