Mouse Macrophage Inflammatory Protein 3alpha,MIP-3alpha ELISA kit

Mouse Macrophage Inflammatory Protein 3alpha,MIP-3alpha ELISA kit

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Mfr.No.

CSB-E04668m

Sensitivity

9.75 pg/ml

Detection Range

39 pg/ml - 2500 pg/ml

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 20, CKb4, LARC, MIP-3a, MIP3A, SCYA20, ST38, exodus-1, macrophage inflammatory protein 3 alpha, small inducible cytokine subfamily A (Cys-Cys), member 20, Macrophage Inflammatory Protein-3alpha,MIP-3alpha, Macrophage Inflammatory Protein-3 alpha,MIP-3-alpha

Recovery

The recovery of human cTn-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	84-110
EDTA plasma (n=4)	96	89-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human cTn-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-100
1:2	Average %	92
	Range %	83-101
1:4	Average %	93
	Range %	85-103
1:8	Average %	95
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.362	2.407	2.385	2.238
1500	1.421	1.453	1.437	1.290
750	0.852	0.846	0.849	0.702
375	0.489	0.512	0.501	0.354
187.5	0.323	0.336	0.330	0.183
94	0.280	0.286	0.283	0.136
47	0.228	0.227	0.228	0.081
0	0.146	0.147	0.147	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O89093

Applications	ELISA
Research Area	CCL20

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10506194	CSB-E04668m	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.