Mouse Macrophage Inflammatory Protein 1beta,MIP-1beta ELISA kit

Mouse Macrophage Inflammatory Protein 1beta,MIP-1beta ELISA kit

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Mfr.No.

CSB-E04665m

Alternative Names

chemokine (C-C motif) ligand 4, ACT2, AT744.1, G-26, LAG1, MGC104418, MGC126025, MGC126026, MIP-1-beta, MIP1B, MIP1B1, SCYA2, SCYA4, CC chemokine ligand 4, chemokine C-C motif ligand 4, lymphocyte-activation gene 1, secreted protein, Macrophage Inflammatory Protein 1beta,MIP-1beta, Macrophage Inflammatory Protein 1 beata,MIP-1-beta

Reactivity

Mouse

Detection Range

12.5 pg/mL-800 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-99
EDTA plasma (n=4)	96	92-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-95
1:2	Average %	89
	Range %	85-94
1:4	Average %	95
	Range %	92-100
1:8	Average %	90
	Range %	85-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.037	1.902	1.970	1.809
2500	1.585	1.583	1.584	1.423
1250	1.184	1.156	1.170	1.009
625	0.821	0.852	0.837	0.676
312	0.595	0.581	0.588	0.427
156	0.372	0.380	0.376	0.215
78	0.249	0.234	0.242	0.081
0	0.164	0.158	0.161	?

Species

Mus musculus

Sensitivity

3.12 pg/mL

Limit Of Detection

450 nm

UniProt ID	P14097
Antigen	chemokine (C-C motif) ligand 4

Applications

ELISA

Research Area

CCL4

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506192	CSB-E04665m	96T	$615	Add to Cart
BHE10506192	CSB-E04665m	5x96T	$2952	Add to Cart
BHE10506192	CSB-E04665m	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.