Mouse Macrophage-Derived Chemokine,MDC ELISA kit

Mouse Macrophage-Derived Chemokine,MDC ELISA kit

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Mfr.No.

CSB-E04661m

Sensitivity

23.672 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 22, A-152E5.1, ABCD-1, DC/B-CK, MDC, MGC34554, SCYA22, STCP-1, CC chemokine STCP-1, macrophage-derived chemokine, small inducible cytokine A22, small inducible cytokine subfamily A (Cys-Cys), member 22, sti

Recovery

The recovery of mouse IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	80-115
EDTA plasma (n=4)	95	90-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	80-99
1:2	Average %	96
	Range %	91-101
1:4	Average %	95
	Range %	92-100
1:8	Average %	98
	Range %	86-115

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.750	2.800	2.775	2.678
250	2.058	2.148	2.103	2.006
125	1.258	1.366	1.312	1.215
62.5	0.785	0.840	0.813	0.716
31.2	0.520	0.482	0.501	0.404
15.6	0.385	0.383	0.384	0.287
7.8	0.196	0.199	0.198	0.101
0	0.098	0.096	0.097	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O88430

Applications	ELISA
Research Area	CCL22

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506190	CSB-E04661m	96T	$495	Add to Cart
BHE10506190	CSB-E04661m	5x96T	$2376	Add to Cart
BHE10506190	CSB-E04661m	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.