Mouse Lysozyme,LZM ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11329m

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Lysozyme,LZM

Recovery

The recovery of rabbit SDF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	93	91-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SDF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	88-106
1:2	Average %	95
	Range %	89-102
1:4	Average %	88
	Range %	85-94
1:8	Average %	99
	Range %	93-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	LZM

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Enhanced antimicrobial activity of engineered human lysozyme /,ACS Chem. Biol,2010 Sep 17;5(9):809-18. Standardized extract of Tinospora crispa stimulates innate and adaptive immune responses in Balb/c mice. Ahmad W. et al,Food Funct.,2016 Feb 3. Inhibitory Effects of the Standardized Extract of Phyllanthus amarus on Cellular and Humoral Immune Responses in Balb/C Mice. Ilangkovan M.et al,Phytother Res.,/

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