Mouse Lysosomal Pro-X Carboxypeptidase

Mfr.No.

E2225Mo

Sensitivity

9.57ng/L

Detection Range

20-4500ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Lysosomal Pro-X carboxypeptidase;PRCP;Proline carboxypeptidase;Prolylcarboxypeptidase

Molecular Weight

55027

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2225Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1747.9	56.84	3.3
Intra-Assay	2	18	422.8	21.7	5.1
Intra-Assay	3	18	918.9	47.63	5.2

Background

Cleaves C-terminal amino acids linked to proline in Peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH (By similarity).

Protocol

These standard curves of E2225Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.184	2.219	2.178
	2.253
1200ng/L	1.379	1.28	1.239
	1.181
600ng/L	0.558	0.649	0.609
	0.741
300ng/L	0.362	0.403	0.363
	0.444
150ng/L	0.145	0.175	0.135
	0.205
0ng/L	0.049	0.04	0
	0.032

Gene ID	72461
UniProt ID	Q7TMR0

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110036	E2225Mo	48T	$320		Add to Cart
BHE12110036	E2225Mo	96T	$458		Add to Cart

Mouse Lysosomal Pro-X Carboxypeptidase - PRCP ELISA Kit

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