Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

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Mfr.No.

CSB-EL018636MO

Alternative Names

prolylcarboxypeptidase (angiotensinase C), HUMPCP, MGC2202, PCP, angiotensinase C, lysosomal Pro-x carboxypeptidase, lysosomal carboxypeptidase C, proline carboxypeptidase, prolylcarboxypeptidase, prolylcarboxypeptidase isoform 1 preproprotein

Reactivity

Mouse

Detection Range

37.5 pg/mL-2400 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of sheep IL-1? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	97	94-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-1? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-90
1:2	Average %	98
	Range %	92-102
1:4	Average %	100
	Range %	96-104
1:8	Average %	94
	Range %	90-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.487	2.456	2.472	2.371
500	2.031	2.000	2.016	1.915
250	1.614	1.568	1.591	1.490
125	1.105	1.023	1.064	0.963
62.5	0.703	0.689	0.696	0.595
31.2	0.421	0.402	0.412	0.311
15.6	0.275	0.262	0.269	0.168
0	0.103	0.099	0.101	?

Species

Mus musculus

Sensitivity

9.37 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q7TMR0
Antigen	prolylcarboxypeptidase (angiotensinase C)

Applications

ELISA

Research Area

PRCP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506182	CSB-EL018636MO	96T	$695	Add to Cart
BHE10506182	CSB-EL018636MO	5x96T	$3336	Add to Cart
BHE10506182	CSB-EL018636MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.