Mouse LYPD3 ELISA Kit PicoKine®

Mfr.No.

EK1838

Sensitivity

<50 pg/ml

Detection Range

93.6 pg/ml - 6,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	93.8	187.5	375	750	1500	3000	6000
O.D.	0.041	0.126	0.194	0.331	0.605	1.025	1.498	2.340

Description

Mouse LYPD3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lypd3 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein C4.4A homolog; Lypd3; C4.4a

Immunogen

Expression system for standard: NS0; Immunogen sequence: L33-S343

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse LYPD3

Subcellular Localization

Cell membrane, GPI-anchor

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	111	913	2985	169	920	2991
Standard deviation	4.66	41.09	152.2	9.97	58.88	224.3
CV (%)	4.2%	4.5%	5.1%	5.9%	6.4%	7.5%

Protein Function

Supports cell migration. May be involved in tumor progression.

Background

Ly6/PLAUR domain-containing protein 3 is a protein that in humans is encoded by the LYPD3 gene. This gene is mapped to 7; 7 A3.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	111	110	102	99	105	5.12	4.8%
Sample 2	913	945	924	872	913	26.57	2.9%
Sample 3	2985	3342	3284	2927	3134	180.84	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	72434
Gene Name	Lypd3
UniProt ID	Q91YK8
Tissue Specificity	Up-regulated in suprabasal keratinocytes of hyperplastic skin induced by phorbol-ester.

Applications	ELISA
Research Area	Cancer,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001409	EK1838	96 wells/kit	$750		Add to Cart

Mouse LYPD3 ELISA Kit PicoKine®

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