Mouse Lymphotoxin-beta - LTB ELISA Kit

Mfr.No.

E1139Mo

Description

This sandwich kit is for the accurate quantitative detection of Mouse Lymphotoxin-beta (also known as LTB) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

LT-beta;Lymphotoxin-beta;TNF C;TNFC;TNF-C;Tumor necrosis factor C;Tumor necrosis factor ligand superfamily member 3

Reactivity

Mouse

Detection Range

7-1500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

32329

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.13ng/L

Background

Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface.

Gene ID	16994
UniProt ID	P41155
Antigen	TNFC

Research Area

Immunology

Protocol

These standard curves of E1139Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.087	1.958	1.905
	1.828
400ng/L	0.975	1.009	0.956
	1.043
200ng/L	0.44	0.504	0.451
	0.567
100ng/L	0.279	0.282	0.23
	0.286
50ng/L	0.121	0.097	0.044
	0.074
0ng/L	0.06	0.053	0
	0.045

Precision

We measured random samples of E1139Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	291	15.11	5.2
Intra-Assay	2	18	532.6	15.9	3
Intra-Assay	3	18	422.6	17.02	4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109005	E1139Mo	48T	$320		Add to Cart
BHE12109005	E1139Mo	96T	$458		Add to Cart

Mouse Lymphotoxin-beta - LTB ELISA Kit

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