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Home / ELISA Kit

Mouse Lupus La protein (SSB) ELISA Kit

SKU : BHE13705422

Sjogren syndrome antigen B (autoantigen La), also known as SSB,La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(OH) elements of newly RNA polymerase III-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and systemic lupus erythematosus (SLE) (Teplova et al., 2006).Grolz and Bachmann (1997) stated that there are 3 La pseudogenes. They also found that alternative splicing gives rise to 3 different mRNAs, all of which were expressed in all tissues tested, and all of which were translated into nuclear La protein in transfected mouse cells.

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Mfr.No.	KTE70262
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Lupus La protein (SSB) ELISA Kit employs a two-site sandwich ELISA to quantitate SSB.
Alternative Names	SSB; LARP3; La; La ribonucleoprotein domain family; member 3; OTTHUMP00000204778; SS-B/La protein; autoantigen La
Detection Method	Colorimetric
Kit Components	Mouse Lupus La protein microplate Mouse Lupus La protein standard Mouse Lupus La protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Lupus La protein (SSB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SSB. No significant cross-reactivity or interference between Mouse SSB and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Sjogren syndrome antigen B (autoantigen La), also known as SSB,La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(OH) elements of newly RNA polymerase III-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and systemic lupus erythematosus (SLE) (Teplova et al., 2006).Grolz and Bachmann (1997) stated that there are 3 La pseudogenes. They also found that alternative splicing gives rise to 3 different mRNAs, all of which were expressed in all tissues tested, and all of which were translated into nuclear La protein in transfected mouse cells.
Notes	This Mouse Lupus La protein (SSB) ELISA Kit employs a two-site sandwich ELISA to quantitate SSB in samples. An antibody specific for SSB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySSB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	20823

Applications	ELISA
Applications Notes	This Mouse Lupus La protein (SSB) ELISA Kit employs a two-site sandwich ELISA to quantitate SSB in samples. An antibody specific for SSB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySSB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705422	KTE70262	48 T	$339		Add to Cart
BHE13705422	KTE70262	96 T	$589		Add to Cart
BHE13705422	KTE70262	96 Tx5	$2629		Add to Cart
BHE13705422	KTE70262	96 Tx50	$24569		Add to Cart

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