Matrices listed below were spiked with certain level of LTC4 and the recovery rates were calculated by comparing the measured value to the expected amount of LTC4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
96
EDTA plasma(n=5)
89-104
95
heparin plasma(n=5)
85-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LTC4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-102%
85-99%
85-102%
EDTA plasma(n=5)
82-97%
84-98%
84-98%
heparin plasma(n=5)
82-94%
83-98%
87-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
LTC4 ELISA Kit allows for the in vitro quantitative determination of LTC4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse LTC4(Cysteinyl Leukotrienes C4) ELISA Kit