Mouse low density lipoprotein cholesterol (LDL-C) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027860MO

Sensitivity

0.078 ug/mL

Detection Range

0.312 ug/mL-20 ug/mL

Reactivity

Mouse

Assay Duration

1-5h

Alternative Names

low density lipoprotein cholesterol (LDL-C)

Recovery

The recovery of sheep GH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-102
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep GH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-101
1:2	Average %	93
	Range %	87-98
1:4	Average %	96
	Range %	95-102
1:8	Average %	95
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	LDL-C

Storage	Store at 2-8°C. Please refer to protocol.

Reference	2,3,5,4'-tetrahydroxy‑stilbene-2-O-β-D-glucoside attenuates methionine and choline‑deficient diet‑induced non‑alcoholic fatty liver disease Mingnuan Han.et al,Experimental and TherapeuticMedicine,June 13

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