Mouse Long-chain-Fatty-acid--CoA Ligase 1

Mfr.No.

E2218Mo

Alternative Names

ACSL 1;ACSL1;Arachidonate--CoA ligase;LACS 1;Long-chain acyl-CoA synthetase 1;Long-chain-fatty-acid--CoA ligase 1;Phytanate--CoA ligase

Reactivity

Mouse

Detection Range

20-3500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

77951

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.02ng/L

Background

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (By similarity). Preferentially uses palmitoleate, oleate and linoleate (By similarity). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs).

Gene ID	14081
UniProt ID	P41216
Antigen	ACSL1

Research Area

Signal Transduction

Protocol

These standard curves of E2218Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.497	2.479	2.442
	2.46
1000ng/L	1.483	1.445	1.408
	1.406
500ng/L	0.774	0.831	0.794
	0.888
250ng/L	0.45	0.414	0.377
	0.377
125ng/L	0.203	0.225	0.188
	0.247
0ng/L	0.043	0.037	0
	0.031

Precision

We measured random samples of E2218Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	618.8	31.46	5.1
Intra-Assay	2	18	1056.8	29.54	2.8
Intra-Assay	3	18	341.3	17.86	5.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110029	E2218Mo	48T	$320		Add to Cart
BHE12110029	E2218Mo	96T	$458		Add to Cart

Mouse Long-chain-Fatty-acid--CoA Ligase 1 - ACSL1 ELISA Kit

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