Mouse Lipopolysaccharides(LPS) ELISA Kit

Mouse Lipopolysaccharides(LPS) ELISA Kit

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Mfr.No.

CSB-E13066m

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Lipopolysaccharides(LPS)

Recovery

The recovery of canine CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-99
EDTA plasma (n=4)	92	87-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-103
1:2	Average %	89
	Range %	84-96
1:4	Average %	97
	Range %	90-108
1:8	Average %	89
	Range %	84-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average	Corrected
20	3.105	3.004	3.055	2.953
10	2.654	2.667	2.661	2.559
5	1.778	1.786	1.782	1.680
2.5	1.026	0.989	1.008	0.906
1.25	0.468	0.478	0.473	0.371
0.625	0.261	0.281	0.271	0.169
0.312	0.158	0.167	0.163	0.061
0	0.103	0.101	0.102	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	LPS

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dietary polyphenols promote growth of the gut bacterium Akkermansia muciniphila and attenuate high fat diet-induced metabolic syndrome Roopchand DE et al,Diabetes,2015 Aug;64(8):2847-58. Lactobacillus rhamnosus CCFM1107 treatment ameliorates alcohol-induced liver injury in a mouse model of chronic alcohol feeding Tian F.et al,J Microbiol.,53(12):856-63 A Modula Helena Cortez-Pinto.et al,GE-Portuguese Joual of Gastroenterology,/ Microbiota Modulation With SynbioticDecreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH) Helena Cortez-Pinto.et al,GE Portuguese Joual of Gastroenterology,2016 10 pages Metabolic Damage Presents Differently in Young and Early-Aged C57BL/6 Mice Fed a High-Fat Diet Liu C Y.et al,Inteational Joual of Gerontology,10(2):105-111. A novel cobiotic-based preventive approach against high-fat diet-induced adiposity, nonalcoholic fatty liver and gut derangement in mice Singh DP.et al,Int J Obes (Lond).,40(3):487-96 Metformin exerts glucose-lowering action in high-fat fed mice via attenuating endotoxemia and enhancing insulin signaling. Zhou ZY.et al,Acta Pharmacol Sin.,/ Metabolic adaptation to the aqueous leaf extract of Moringa oleifera Lam.-supplemented diet is related to the modulation of gut microbiota in mice Gao X.et al,Appl Microbiol Biotechnol.,Apr 5. Effect of miglitol on the suppression of nonalcoholic steatohepatitis development and improvement of the gut environment in a rodent model Kishida Y.et al,J Gastroenterol. ,Mar 27. Isomalto-oligosaccharides, a prebiotic, functionallyAugment green tea effects against high fat diet-induced metabolic alterations via preventing gut dysbacteriosis in mice Singh DP.et al,Pharmacol Res.,Jun 28 Bixin ameliorates high fat diet-induced cardiac injury in mice through inflammation and oxidative stress suppression Xu Z.et al,Biomedicine & Pharmacotherapy,Mar 10;89:991-1004. Arachidonic acid sex-dependently affects obesity through linking gut microbiota-driven inflammation to hypothalamus-adipose-liver axis Zhuang P.et al,BBA-MOL BASIS DIS ,Jul 12 Adiponectin administration alleviates DSS-induced colonic inflammation in Caco-2 cells and mice Qin Zhao.et al,Inflammation Research,May 15 Fungal polysaccharopeptides reduce obesity by richness of specific microbiota and modulation of lipid metabolism Xiaojun Li.et al,bioRxiv ,July 24 Flavor compounds in fermented Chinese alcoholic beverage alter gut microbiota and attenuate ethanol-induced liver Cheng Fang.et al,bioRxiv,Octobert 8

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BHE10506152	CSB-E13066m	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.