Mouse Leukocyte surface antigen CD47(CD47) ELISA kit

Mouse Leukocyte surface antigen CD47(CD47) ELISA kit

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Mfr.No.

CSB-EL004940MO

Alternative Names

CD47 molecule, IAP, MER6, OA3, CD47 antigen, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, Rh-related antigen, antigen identified by monoclonal antibody 1D8, antigenic sur

Reactivity

Mouse

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse Desmosine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-96
EDTA plasma (n=4)	104	96-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Desmosine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:10	Average %	85
	Range %	81-92
1:20	Average %	96
	Range %	91-103
1:40	Average %	103
	Range %	97-107
1:80	Average %	98
	Range %	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.495	2.594	2.545	2.355
100	1.804	1.903	1.854	1.664
50	1.128	1.148	1.138	0.948
25	0.670	0.661	0.666	0.476
12.5	0.441	0.422	0.432	0.242
6.25	0.314	0.305	0.310	0.120
3.12	0.255	0.257	0.256	0.066
0	0.189	0.190	0.190	?

Species

Mus musculus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q61735
Antigen	CD47 molecule

Applications

ELISA

Research Area

CD47

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506140	CSB-EL004940MO	96T	$695	Add to Cart
BHE10506140	CSB-EL004940MO	5x96T	$3336	Add to Cart
BHE10506140	CSB-EL004940MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.