Mouse Leukemia Inhibitory Factor

Mfr.No.

E0645Mo

Sensitivity

0.046ng/mL

Detection Range

0.1-40ng/mL

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Leukemia inhibitory factor (also known as LIF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

D factor;Differentiation-stimulating factor;Leukemia inhibitory factor;LIF

Molecular Weight

22287

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0645Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.2	0.41	7.9
Intra-Assay	2	18	14.5	0.74	5.1
Intra-Assay	3	18	1.2	0.09	7.3

Background

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Protocol

These standard curves of E0645Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	2.195	2.199	2.149
	2.203
12ng/mL	1.654	1.616	1.566
	1.579
6ng/mL	1.114	1.045	0.995
	0.976
3ng/mL	0.724	0.75	0.7
	0.777
1.5ng/mL	0.476	0.466	0.417
	0.457
0ng/mL	0.038	0.05	0
	0.062

Gene ID	16878
UniProt ID	P09056

Research Area	Cancer

Storage	2-8°C

Reference	Higher endometrial receptivity caused by Letrozole in antagonist protocol-stimulated mouse uterus Journal: Bali Medical Journal 7(2): 369-378.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12108523	E0645Mo	48T	$320		Add to Cart
BHE12108523	E0645Mo	96T	$458		Add to Cart

Mouse Leukemia Inhibitory Factor - LIF ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse Leukemia Inhibitory Factor - LIF ELISA Kit

Rate it now..!!