Mouse Leukemia inhibitory factor,LIF ELISA kit

Mfr.No.

CSB-E04652m

Alternative Names

leukemia inhibitory factor (cholinergic differentiation factor), CDF, DIA, HILDA, D factor, cholinergic differentiation factor, differentiation inhibitory activity, differentiation stimulating factor

Reactivity

Mouse

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human Bcl6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	87	83-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Bcl6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-103
1:2	Average %	100
	Range %	97-104
1:4	Average %	86
	Range %	83-89
1:8	Average %	98
	Range %	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.790	2.701	2.746	2.588
600	2.158	2.032	2.095	1.937
300	1.269	1.304	1.287	1.129
150	0.784	0.811	0.798	0.640
75	0.516	0.531	0.524	0.366
37.5	0.342	0.336	0.339	0.181
18.75	0.246	0.252	0.249	0.091
0	0.157	0.159	0.158	?

Species

Mus musculus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P09056
Antigen	leukemia inhibitory factor (cholinergic differentiation factor)

Applications

ELISA

Research Area

LIF

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506136	CSB-E04652m	96T	$835	Add to Cart
BHE10506136	CSB-E04652m	5x96T	$4008	Add to Cart
BHE10506136	CSB-E04652m	10x96T	$7932.50	Add to Cart

Mouse Leukemia inhibitory factor,LIF ELISA kit

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