Mouse Leptin ELISA Kit

Mfr.No.

EM0129

Alternative Names

Leptin ELISA Kit, LEP ELISA Kit, LEPD ELISA Kit, OBS ELISA Kit, Obesity Homolog ELISA Kit

Reactivity

Mouse

Detection Method

Double Antibody

Detection Range

62.5-4000pg/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Leptin/LEP/LEPD/OBS/Obesity Homolog and the recovery rates were calculated by comparing the measured value to the expected amount of Leptin/LEP/LEPD/OBS/Obesity Homolog in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-104	96
EDTA plasma(n=5)	89-103	99
heparin plasma(n=5)	87-105	99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Leptin/LEP/LEPD/OBS/Obesity Homolog and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	98-103%	88-105%	88-100%
EDTA plasma(n=5)	83-99%	82-97%	88-94%
heparin plasma(n=5)	85-99%	81-96%	82-99%

Sensitivity

< 37.5pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Leptin/LEP/LEPD/OBS/Obesity Homolog

Notes

For Research Use Only

UniProt ID	P41160
Antigen	leptin

Applications	ELISA
Applications Notes	Leptin ELISA Kit allows for the in vitro quantitative determination of Leptin/LEP/LEPD/OBS/Obesity Homolog concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: American Journal of Translational Research Title: Obesity attenuates force-induced tooth movement in mice with the elevation of leptin level: a preliminary translational study

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SKU	Supplier No.	Size	Your price	Quantity
BHE10803741	EM0129	96T	$350		Add to Cart

Mouse Leptin ELISA Kit

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