Matrices listed below were spiked with certain level of LECT2 and the recovery rates were calculated by comparing the measured value to the expected amount of LECT2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-99
94
EDTA plasma(n=5)
86-105
95
heparin plasma(n=5)
85-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LECT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-100%
85-101%
85-102%
EDTA plasma(n=5)
84-98%
87-96%
83-92%
heparin plasma(n=5)
92-99%
81-99%
87-97%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
O88803
Applications
ELISA
Applications Notes
LECT2ELISA Kit allows for the in vitro quantitative determination of LECT2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse LECT2(Leukocyte cell-derived chemotaxin-2)ELISA Kit