Matrices listed below were spiked with certain level of Leap2 and the recovery rates were calculated by comparing the measured value to the expected amount of Leap2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
94
EDTA plasma(n=5)
93-105
100
heparin plasma(n=5)
86-105
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Leap2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-98%
86-104%
88-105%
EDTA plasma(n=5)
90-101%
85-98%
92-98%
heparin plasma(n=5)
87-100%
87-95%
86-97%
Sensitivity
< 9.375ng/ml
Notes
For Research Use Only
UniProt ID
Q91V13
Applications
ELISA
Applications Notes
Leap2 ELISA Kit allows for the in vitro quantitative determination of Leap2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Leap2(Liver-expressed antimicrobial peptide 2) ELISA Kit