Mouse Laminin,LN ELISA kit

Mfr.No.

CSB-E04645m

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Laminin,LN

Recovery

The recovery of pig T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	97	93-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pigT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	98
	Range %	87-105
1:4	Average %	92
	Range %	85-100
1:8	Average %	95
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	LN

Storage	Store at 2-8°C. Please refer to protocol.

Reference	SIRT3 Facilitates Amniotic Fluid Stem Cells to Repair Diabetic Nephropathy Through Protecting Mitochondrial Homeostasis by Modulation of Mitophagy Feng J.et al,Cellular Physiology and Biochemistry,April 19

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