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Home / ELISA Kit

Mouse Klotho (KL) ELISA Kit

SKU : BHE13706368

Klotho is a type-I membrane protein that is related to beta-glucuronidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.
Klotho is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. Its discovery was documented in 1997 by Kuro-o et al. The name of the gene comes from Klotho or Clotho, one of the Moirae, or Fates, in Greek mythology.

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Mfr.No.	KTE71209
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Klotho (KL) ELISA Kit employs a two-site sandwich ELISA to quantitate KL.
Alternative Names	KL
Detection Method	Colorimetric
Kit Components	Mouse Klotho microplate Mouse Klotho standard Mouse Klotho detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Klotho (KL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse KL. No significant cross-reactivity or interference between Mouse KL and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Klotho is a type-I membrane protein that is related to beta-glucuronidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. Klotho is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. Its discovery was documented in 1997 by Kuro-o et al. The name of the gene comes from Klotho or Clotho, one of the Moirae, or Fates, in Greek mythology.
Notes	This Mouse Klotho (KL) ELISA Kit employs a two-site sandwich ELISA to quantitate KL in samples. An antibody specific for KL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	16591

Applications	ELISA
Applications Notes	This Mouse Klotho (KL) ELISA Kit employs a two-site sandwich ELISA to quantitate KL in samples. An antibody specific for KL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13706368	KTE71209	48 T	$339		Add to Cart
BHE13706368	KTE71209	96 T	$589		Add to Cart
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BHE13706368	KTE71209	96 Tx50	$24569		Add to Cart

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