Matrices listed below were spiked with certain level of Kiss1 and the recovery rates were calculated by comparing the measured value to the expected amount of Kiss1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
95
EDTA plasma(n=5)
93-102
97
heparin plasma(n=5)
88-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kiss1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Kiss1 ELISA Kit allows for the in vitro quantitative determination of Kiss1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Kiss1(Metastasis-suppressor KiSS-1) ELISA Kit